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Uploaded by Admin on Jan 22, 1999

Introduction: Carbohydrates are molecules that contain Carbon, Hydrogen, and Oxygen. The most basic sugar- carbohydrate is the monosaccharide. Carbohydrate molecules are categorized by the number of carbons present in the molecule. Glucose, the most common monosaccharide has six carbons per molecule, which is called a hexose. Carbohydrates also have a 2:1 hydrogen to oxygen ratio. This aids in the condensation and hydrolysis reactions. A condensation reaction occurs when two monosaccharides join by the removal of H2O. During condensation synthesis one monosaccharide losses an OH and the other losses a H. As a result the two monosaccharides bond by forming maltose a disaccharide with a by-product of a free H2O. When three or more monosaccharides or monomer are involved in a condensation synthesis a polymer or polysaccharide is formed with a by product of water. Starch and Glycogen are two common chain polymers. Just as the removal of water joins carbohydrates together via condensation synthesis, hydrolysis is the reaction using water to break apart Di and polysaccharide. A Di-saccharide is bound by sharing an oxygen with one hydrogen on each opposing side. When H2O is re-introduced via hydrolysis the two monomers in the disaccharide no longer need to share one H2O, and as a result the opposing sides absorb the OH and H and break apart. This reaction is a common theme in the digestion of complex carbohydrates. Experiment 3: Test for Sugars We will test five different solutions for presence or absence of reducing sugars using a premixed Benedict's reagent. These dependent variable solutions will be water, glucose , onion juice, potato juice, and starch suspension. When the Benidict's reagent is combined with sugars at a boiling temperature, a color reaction will occur. Our independent variable, Benidict's reagent color is blue. The negative control will be the combination of water and Benidict's reagent. This sample will undergo all the steps of the test except for the one being tested for i.e. sugars. If the end result is negative color change (blue) , it will thus be determined that the experiment and the Benidict's reagent is not contaminated and the experiment may proceed. The negative control will also serve as a comparison to gauge against the color change in the other four sugar tests. EXPERIMENT 3: Test for Sugars: TESTS MAT. & METHODS HYPOTHESIS RESULTS Tube 1 1cm water+2cm Benidict's reagent. No color change Negative control no color change. Neg-sugar. Blue Tube 2 1cm glucose solution+ 2cm Benidict's reagent. Boil...

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Uploaded by:   Admin

Date:   01/22/1999

Category:   Science And Technology

Length:   6 pages (1,294 words)

Views:   2379

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